ehT encpcot si a cnoedluvto wya fo aingsk if uoy nkwe owh DJV itionencrabom s,wkro which is htta ti si cylatlau an mplexae fo neagtrli eht ADN of eht TB/ plt.cemhyoy

tueSrnho lobt iuenqce:th oS ewhn hety use a pbore agsanti some ionge,r adn piugunottt a iesz fo 15. bk or 6 ,kb ihst is ntellgi oyu eht ezis fo teh NDA aeftnmgr ni aehc ellc (ensto’d mettar if yhet ysa J eorpb or ttcnoasn eorgni ebp,or tyhr’ee just sgnaiy h’rytee tenragitg smeo neclutedoi ceseunqe dnufo in the Ig lTcuC/soR taeb hcani scluo vyteeielprsc fro BT/ l)csl.e

I hnikt eth ongiuncfs rpta ludco be ornwnedig who uoy owkn hheetwr uoery’ ayrlpt tgruohh egnarnrtaeemr rwsea(n oiscehc B ruht )D or fi ti ntha’s dcrruoec ta lal yet c(rctero anr.)ews e,Her eth cnotcpe si that B eclls drneoug (J)DV trneenaermgra ni hte enob wrar,mo iwelh T cslel od ti in het sht,umy nda it lla shpnpae ta .neco So a mlsapa ecll in het bdolo leik ni Mtilepul meyoaMl doluw eahv uylfl nruodngee nemiora,nbctoi wiehl a T lelc in hte dbloo duclo ehrite be flyul ceeddaut (dan vaeh ifsedihn DJV btnoma)cniiore or tuiamerm htna(’s tedasrt D)J.V

eSicn the T lecl gene was 6 bk and ndtylefeii rgebgi hnat the 1.5 bk een,g eht T clel hnsat’ nunerodge eacntominrbio et.y

orSth nsreaw: llA ngees rae reentsp in tbho B nda T cles,l tub eyht nrgaeaerr adn epxsers prsotcree ahtt rae peciicfs ot irhet le.neiga ehriT ear nnddetnepie JVD igenors rof B elcl ctreoper orintganee dan T lelc eotprerc nignt!aoeer hTsi wkii caiterl hsdse thilg on :tish

rde_ile/bi/ip.pl:#V/ikoasntekt_ghi.twomcsprTe/eic)rioo_ntDrncewJ(a

I oto clagryailt emisds .th.si tbu ti si aulaytcl so simpel atht we era gsmniis eht tsroef trhuohg eht teres -lhe W-i tohb B dan T sclle ugodenr DJV t,egaamnrreern the V, D, nda J gnsee ear DFRIETNEF EESGN pdeties nbgei ioenrdagz dna rthorefee flcissaedi ni a lirmsia fionhsa deu ot ehitr areshd ened to tnearege vresied rrsseutuct. To mkae mtesrat meor g,unnsifoc bhto tsse fo VDJ gsene rea on mroomehcos .41

iTsh si hyw eth EBMN deiisfepc thta eyth ear atgggni ETH EASM J ENROIG iwhch is thta fo eht B cell tperreoc neeg J gioern, not teh T lelc reeocrpt ngee -Jetab gonrie es(e iwik a)etci.rl ehT B lcel rcpoteer egen is ont errndgaaer ni a T ,elcl fi it w,as T llsec wdoul vaeh beasoniti!d

Teh wya I guohhtt btoua shti nesotqui was ttha ni MM heter si a TON of isdieatnbo gbine e,dam so teh DJV smegten si geibn nekorb dpcregdrt/slneereeaua/ yamn estmi nda ahs enbe tnsehrdoe ot b..51k sA ofr eht T l,Cesl htta orneig ’snti biegn dsue inse(c rtehe si no llocan onnxpisae ro )eoetcsiln so tis’ ilslt tgo eht ufll kb6 ntlhge .uncoehudt

ehiWl T lescl od ues het DVJ cbm,roianeinto eth T lecl ertcopre snhaic aer fdntirefe sgeen mrof eht B eslcl' heayv nda glhit h.iansc cinSe yhet esud a B lecl rgoJein- obpe,r teh T sc'lle bnda wlil be umch gerlra sa hist arae fo tis DNA the( B clle hyaev or glhti hniac ns)eeg aevh not eneb rrdenregaa -- tdnesia sit T ellc retcepor gseen aveh eebn aerrndgr.ea

_apohpnm_m/tr/_p//pztqoenn2nstrsiebe_epeoi7t_be/tteilmraelq42duo:wwhbde//nscumwrxlsce.i.ts_t/ns1o