o,S fro smoe e,anors osttutnnrmseriaer is egtnshmoi eIv' ALAYSW erdggslut ht.wi.. rylobapb eceubsa I na'wst thgaut it llew so vrnee yaller eneradl it, ustj nedlrae genuho for ertaevhw m.axe I tsju nfudo shti oob htoguhr ICBN nad tis TNFSTAICA! st'I mrfo 1002 os mghti be d,ol tub ti swa ret.ag You acn crashe uhhogtr hte bkoo dna dinf teh hsta.prec I tepryt uhcm sjtu tnwe ohgrhtu lla fo het rreosiuentarnmtt rh.tacpe m.ho:B0o1t.w/K/hnn/bgpv7wts/sNio.nbli9ck/w9.

My mian ua y:eGt-wasltaeakmta = aomrj yiaetrxcto rtrtsniuoatmrn.ee Tow setyp fo 1:rtoesrpec) bocermtiaop,t smto of hhiwc ear crpapsnieyt Gi hciwh sdela to aeerdsedc DAMN retoerpc tvciatiy dna sirk of i,oytitectioxcxi or anspitscoytp Gq eprstcero ttah adel to cnsaerie ,+Na ,+K and eedercsda amegttual usaesc zranoptloiedai nad ineasercd ++Mg ndpsctealeim dan DNMA rteeorcp aiticvty nad iksr fo ccitt,oxxteoyii dan )2 tcpioorino nhsceanl gndlnuici DAMN dan PnAiMeaa/tAk esnlhcna, chiwh all wloal npiconsfcie naiotc unx,lfi btu ynol NDAM allswo aC++ xiulfn d(an nylo in a aveoltg ddpetenen earnmn faert setniuffic oelirptzdinaoa hsa peacdisld teh oirntibih Mg++ noi in eth )enha.cnl

AAGB dna cnegyil = iyorbhtini ttreairo.snsuetmnr 1 ) GBA-AA adn -GACAB = ooprctiino nhsancel atth aeld ot felxu fo C-l, nad ptesdei shti gcaunis eipaaid,lrotonz het enourn tlils sasyt olewb geitrsn nopailett. AAAB-G ibidnng itse rof rta,ebrsbauti s,sotedir ,GBAA dan xiocopnrti = edsiin opre fo nehal.nc BAAGA- nidngib tsie of szeainbeoeizdpn = dosietu of orpe fo hcnlean. 2) niGecly lhnanec si a vrey isrlami -lC ulefx enhncla. 3 ) -BAAGB si a mopbecroatit cennalh ttha evcittasa Gi ldgneia ot ecdeedasr PcMA icwhh tiaecstva feufxl +K chesnlna nda thsiibin +a+C lnuxif ehcnnsal glendia ot rryhzlaopepoi.tain

Bgicnoei isamen = ochcamlstneiea pdaoemin nacodrooniit( fo oybd enom,emtv adwrer, otoniima,vt erermt,inocn)fe pinneienoehrpr lpese(, esfuweak,nsl attntn,ieo gdefien iaeh,rovb inrpeinpehe tws(leo eotntrnacncoi in N,S)C lpsu stoienron ee(lps, asnlf,esekwu enspdr,osie an,xitey enaasu) and tihsnamei u,roasl(a ,oatteitnn l,ealygr usties ea,amdg dna aym cuienfnel lobod nirab .l)fwo uvsoy,lOib all of sthi si in tdndiaoi to aengrcedri nursoseriionmntsa dna gilt,fh r,fgith dan hfgit rsp.eenos

APT adn orhet ieunsrp = ytiaroxtec instnsoasrim, -lseaedrceo whit otrhe lammlulleosce- ru.rstarnoemsnteit dionenAse 'ntis ysilscalalc cddoeienrs a rmstroetnruaietn aescube ti nst'i edes/raldeerost in a++C ndpenedte ,anmnre but derevdi from ATP bfreeo hnaigv an tayrteioxc i.leottapn

neielActhyocl = ojmra rittnnearotmures vdviolne in nisrsoenianurostm aiv anrmisuc adn occtniiin ctore.sepr

etPedpi unnromtrrsetsitae = cnommoyl esealdre sa idtproepep raelgr esrsroprcu htat rae eadevlc by ficepisc emynsze atht wree in hte aems ritrseurtonematn cvsleei unpo aeeesrl. Feiv yetps = g/uantrbi ,pesptide oiopdi s,teipped ytrptuiai tsp,pdei hoiacplhamty asenilger hnom,oser adn shoet nto sii.lcasdfe aEpsxeml = rorscuerp thta igsev seri ot csneubtsa P p,oai(sppumhc oc,oenexrt adn IGT dna ldaseere fmro llasm iadermte SNP C sibefr atht nasrtitm inpa nda uepemraetrt ,fornoaimitn lwrofpeu e,ptnhioevsy htdebiini yb ioipdo te)esdpip, knorinnuie ,A tuerdioepnpe K, adn epdurepitone ,maamg adn pdoioi sietpepd cnulgidni pnalt laldokais ek(il mr,i)noehp ntihectsy ioipdo rtviavsde,ei nda e,spidhonnr nhorins,dpy nad pslknanh.eie In eaenrgl oiidpo deipptse aer nsrstdsapee .e(.i ealaiasng ms),hancemi ildonvve in olmepxc vhoesrbia se(luax arn,ttacoit sisrsgi/bievauevgssem aioe)hs,brv adn impicldaet g(huoth otn eendtifiv)i in pyrhasictic rriseds.do

re,aOllv ietasrrtsrnutnemo = ptye tsye:p -lomucalelemls assmrrntitte dna rie,dtpspeeuon rewhe mole-laluselmsc trsritsamnte rae eafsrt nad dmteeai aprid ispntyca trionsisasmn (.ie. gerndano SNS hgh/thfigrtf/flgtii ckiuq ,seop)rnse rwhee sa uoeedesntripp ngloa( itwh ciigolob mnaies adn oems lamsl luomclee tantst)mirrse rea lewros adn dmeaeti ar,ugdal dlroenogp resmnsisi.rooatnun

eSom scertuip: eoest/erdr.rtfaudunnkoophetwe:dehmioup/lvviwthp_arctk.ebwe/nmn_t/r.upmtas/_iis, lr1:id/ogaeitfn8crger-r06orpwhw/-TsaeohP/1-3.mbw/n0b--s/ieel60gfc/es0na4tat-sbl36rauR3u0f961.Bpead1isdAas51eAe-t4iButr8ioGpctp22cph7d34-/bfgg2areer, and _/-g2x.:daghdeslepfurUxerb/.-ga-wtelc;iob1m6=ta-03leda--a81/5edhbf1b6.w#bi4/5ade7en1ewrp/fgnwh&g=ai--fast90s/2nt3til

guoTh ietnso.qu

aRlelc ahtt mMnieante l'mzeas(eihr udrg) si a DNAM trepReco Anisngtota htat eshpl enerptv ytoicctxoteix by cgknBoli aC2+ nteyr. 'tTsha how I mermeber thsi.

• )A AGAB-A trereopsc tle -Cl in
• B) eiGclny si ueds ni teh plnais rodc sa na nbirhiioty ttnra;ienestmrour osla estl -lC in
• C dan E) Moioebatptcr eamultgat tcrporsee rea per-Gotni eudpolc
• ,Troehreef the lony hgnit that uldco elt Ca+2 ni si DMNA sperotrec

NADM oprecrets in iths aesc ear being eusd rof olgn emtr tnotoapitein nihtwi het barin nda tou of all of teehr ohicecs nyol stih cretrpeo esus Ca+

uYo can ewsarn yb rsosecp fo imenli.notia timoCeveit"p irntia"ctnsoe emksa uyo hinkt oauryitmtls TN. Cossr uto GAAB adn ecniy.gl In hte roectx so u.emlagatt obtcoipetraM woudl amen h'ertse cdsone geemssrens vndivole adn het tprecroe wodul ont trasmtin ccla.imu eecnH .NADM

udse in glon emrt anpttoieoint neulaga,(g )mo,ermy yMa olsa paly a roel ni eegn ncaorittpirns ni neurons

1) DANM is a maautleGt rercopet eyupst2)b MPAA is a tpyesbu fo AMND htta si inllatiyi ecaditatv yb elutgtaam 3eee)sl ra ADNM esotrcepr rea ndofu aern APMA ptrroesec tub are ton viettacda by low ellevs of aaultmtge seeeral edu ot a Mg ion lnbkoc’‘gi the hnaelc) n4 qeentrFu onictA titnalpoe t&g;— asnoliimtut of APAM gt;&— oledszriape tsop ictpaysn enruno t;&g— elagovt dedepnnte gM lcbok fo ADMN reorcetp meoderv )5 nfuxli of aC ohtuhrg NMAD ;gt&— seucas eevn more PAMA esporctre to eb irdeetns oitn eht nmbeamer O:NTE( PAMA si pviernesos to aC adn glua6) am)tte eseBuac there era emor esrtrepoc to posedrn ot it, teh lcel is roem vesnporsie ot ueamttlag

lal het esorht a pvtneomnectioi insce etyh ahve sti(iiifpifscyeeccpc lsi,ga)nd NADM si het yoln nceslnv(eneothieec mepeivitoct cetorre,)p hsa a gialnd seti nad ac , na and .eec.mhgn. epcmtiotiev