NBME 23 Answers : Block 1 : #16 = Physical analysis of the isolated genomic DNA from ...

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NBME Answers & Explanations

NBME 23 Answers

nbme23/Block 1/Question#16

Physical analysis of the isolated genomic DNA from ...

Methylase

+5 upvote downvote
submitted by thomasalterman(94),

Methylase methylates DNA, making the DNA resistant to restriction endonucleases

*https://d2jmvrsizmvf4x.cloudfront.net/7Pf4zZ3TuGwLTRKvUGYL_methylatedrestriction.jpg

+3 upvote downvote
submitted by defalty98(2),

Why are we complicating things? Change in the bases will destroy the palindromic sequence required for any restriction endonuclease to work. Methylation is the only option that makes sense.

arcanumm This makes sense have reading what your comment. I overlooked this and just assumed the GATC was a mutation that allowed the restriction enzyme to work on the mutant only. +

arcanumm it makes even more sense when looking at "numerous small fragments." Methylation is truly the obvious answer here in retrospect. +1

bgiri DNAse can also cause a change in base by breaking down dna at the GATC sequence? +

+3 upvote downvote
submitted by stinkysulfaeggs(34),

Me reading this question stem: .....do you mean which of the following ENZYMES?

+1 upvote downvote
submitted by imgdoc(53),

Explanations for this are too complicated. Think of it like this:

You've got a piece of mutated DNA that is able to be digested by a restriction endonuclease, that means the DNA was transcriptionally available to begin with. AKA it was not methylated, because as we know, methylation = heterochromatin which is transcriptionally inactive. that means methylase was mutated

Only other plausible answer was DNase, and if it was mutated it would be inactive, not overactive.

+0 upvote downvote
submitted by consistentwrongdoer3(9),

Why does methylation cause loss of resistance to GATC restriction endonuclease? Does this have to do with methylation of U to T?

methylased GATC related to methylase --> https://en.wikipedia.org/wiki/Dam_methylase +3

sympathetikey Dam methylase, alright +2

yogi https://www.neb.com/tools-and-resources/usage-guidelines/dam-and-dcm-methylases-of-e-coli +2