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NBME 23 Answers

nbme23/Block 1/Question#16

Physical analysis of the isolated genomic DNA from ...


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Methylase methylates DNA, making the DNA resistant to restriction endonucleases


Me reading this question stem: .....do you mean which of the following ENZYMES?

Why does methylation cause loss of resistance to GATC restriction endonuclease? Does this have to do with methylation of U to T?

methylased  GATC related to methylase --> https://en.wikipedia.org/wiki/Dam_methylase +2  
sympathetikey  Dam methylase, alright +2  

Why are we complicating things? Change in the bases will destroy the palindromic sequence required for any restriction endonuclease to work. Methylation is the only option that makes sense.