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NBME 24 Answers

nbme24/Block 4/Question#31 (46.9 difficulty score)
In a study of antibiotic resistance, a strain ...

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submitted by lilmonkey(18),
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yoTda I rleenad taht frtfenide lairleb icsepse of bitacare cna veha jon.oiatnguc oS naty.s

Gram negative rods are known for their ability to transfer drug resistance via plasmid transfer during conjugation.

+3/- olalala(3),

submitted by medskool123(29),
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anc seooemn exnlpai yhw siht si ont natt?nsucroid Last nemb I dsia aojiotnuncg nad gto ti onrwg fro rost..nctnadui this oen I yas isuatrnnocdt adn tsi gtajon.nicuo

pseudorosette  I would say because this happened between two bacteria, but in transduction what causes the acquisition of bacterial resistance is coming from a bacteriophage, which is a virus that infects bacteria, but that is never hinted at the question! +4  
medpsychosis  Quick Overview of the involved topics and answer choices that are relevant in this question: Transduction: Involves phage, cleaves DNA and takes a part with it as it is packaged. Generalized is when is happens by accident. Specialized is an excision event. Transformation: bacteria takes up naked DNA around it and incorporates it therefore becoming "transformed" e.g. (SHiN) S. Pneuma, H. Influenza type B, and Neisseria. Transposition: Jumping from one location to another within same bacterial organism (e.g. from chromosome to plasmid) Conjugation: Above mentioned plasmid gets transferred from conjugal bridge from one bacteria to another. +16  
wowo  FA2019 p130 +1  
zbird  Easy here...first both are G-ves which likely have a sex pilus and if cultured together as in this case transfer their plasmid. Transduction need phage. Transposition is exchange of genetic material inside the bacteria b/n the dna and the plasmid or vv (FA2019) +  

submitted by medstudied(1),
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anC meooens pienxal wyh eht rccoter rnasew rof hte qteosinu hree si njagocuiont tbu t’nac be ?onosrtitanips

catacholamine16  Transposition is when a segment of DNA (in this case, coding for resistance) jumps onto a plasmid within the same bacterial cell. That plasmid might then transfer to another nearby bacterial cell via conjugation. Transposition is happening WITHIN the bacterium. Conjugation is how that resistance gene gets transferred. +12  
lsmarshall  Also, E. coli is the classic example of a bug tat uses conjugation. ^but explanation above is correct^ +2  
seagull  I think he might have did what I did. I got Transformation mixed up with transposition. FML +3  
luciana  I still can't understand why it can't be transduction. Is it just because of bacterial types? +  
thotcandy  @luciana Yes, I believe so. You have to remember which bacteria have a conjugation pilus - E. coli is the most popular one because of its F sex factor (remember the F+ x F0 thing in FA?) +  
mgemge  I was also confused why it's not transduction...but simply as a crappy memory pneumonic TranNsduction TraNSfers ToxiNs FA 2020 p130 +