NBME 24 Conjugation (NBME Answers)

NBME 24 Answers ↦

nbme24/Block 4/Question#31 (47.9 difficulty score)
In a study of antibiotic resistance, a strain ...
Conjugation🔍,📺
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+3
submitted by lilmonkey(19),

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Gram negative rods are known for their ability to transfer drug resistance via plasmid transfer during conjugation.

+3/- olalala(3),

+1
submitted by medskool123(29),

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pseudorosette I would say because this happened between two bacteria, but in transduction what causes the acquisition of bacterial resistance is coming from a bacteriophage, which is a virus that infects bacteria, but that is never hinted at the question! +4

medpsychosis Quick Overview of the involved topics and answer choices that are relevant in this question: Transduction: Involves phage, cleaves DNA and takes a part with it as it is packaged. Generalized is when is happens by accident. Specialized is an excision event. Transformation: bacteria takes up naked DNA around it and incorporates it therefore becoming "transformed" e.g. (SHiN) S. Pneuma, H. Influenza type B, and Neisseria. Transposition: Jumping from one location to another within same bacterial organism (e.g. from chromosome to plasmid) Conjugation: Above mentioned plasmid gets transferred from conjugal bridge from one bacteria to another. +17

wowo FA2019 p130 +1

zbird Easy here...first both are G-ves which likely have a sex pilus and if cultured together as in this case transfer their plasmid. Transduction need phage. Transposition is exchange of genetic material inside the bacteria b/n the dna and the plasmid or vv (FA2019) +

+0
submitted by medstudied(0),

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catacholamine16 Transposition is when a segment of DNA (in this case, coding for resistance) jumps onto a plasmid within the same bacterial cell. That plasmid might then transfer to another nearby bacterial cell via conjugation. Transposition is happening WITHIN the bacterium. Conjugation is how that resistance gene gets transferred. +12

lsmarshall Also, E. coli is the classic example of a bug tat uses conjugation. ^but explanation above is correct^ +2

seagull I think he might have did what I did. I got Transformation mixed up with transposition. FML +3

luciana I still can't understand why it can't be transduction. Is it just because of bacterial types? +

thotcandy @luciana Yes, I believe so. You have to remember which bacteria have a conjugation pilus - E. coli is the most popular one because of its F sex factor (remember the F+ x F0 thing in FA?) +

mgemge I was also confused why it's not transduction...but simply as a crappy memory pneumonic TranNsduction TraNSfers ToxiNs FA 2020 p130 +