heT ivweenLear ukrB oPlt is lmaiyn tpierrnedet yb tis X adn Y Tctrensp.t ehie tqioesun tatses that givngi B6 aesciersn hte yivatict ot oamrnl sellev, wichh aesnm het ciyivatt uoshld eb the msae sa eht oemaHnlnce. r, eriht Y tpecnreit usldoh eb het ,sema os we aer wtnebee hoiscec B dan .C A dan D do otn avhe het mesa Y ttpenerci sa mn .raerihlogH ncsiretnotoacn fo B6 esduac ivcaitty to bcoeme ,oanrml dna so xamV ilwl tno eb ngniagch dna ew acn acncel A nda .AD has a rwelo mVxa nda D sha a mcuh hgeirh . aVeTmhx efedfrcein ebwtnee B adn C si in hteri mK. Mogivn to het etlf on the X axsi smeka eht Km ewlro nad ltsel you that fftnaiiy si eih hrosg yuo oudlw ton ende rmeo B.6 uBt in oru esac iyfatnif of teh ynzeme ofr B6 si llaeyr wo,l ihchw si hwy we ende a nto rmeo .B6
In usa,mymr we awnt teh esma xaVm nda a rghieh .W Kme wtan eht manr""ol tvaiycti sme(a mvxa sa )rnmlao adn we dene irhhge osamnut of B6 for esuccss f tfoiisany fo het yenmez ofr B6 is pryblaob ervy ol.w
Ciohec B sha a -/1Km)( aulev slcoer to 0 cwihh smane Km is lorew dna ffainiyt of eht mzynee rof its rbestusta si rspue ilsT.o hw seakm ensse sa gginiv ehrihg musanto fo the toceemptv"ii" bssautetr 6B is .einhlpg
niNeedg h"hreig csnni"cootteanr of teh 6B for emzney tyaiticv si netaohr way of asygin Km si hiregh since mreo si erurdqei fro 21/ mvxa aicti.tvy daeIesncr mK ulvsea treusl ni /1mK bigen lmaselr lrceso( to 0 no teh )xi,xas- iwchh is nodedtartesm in srneaw iohecc .B
A na"r"lmo nezyme ctiaiytv in het psnereec fo erhigh B6 nritcncaooetns ansem htta xamV is tno igha,gnnc but Km si.
moeS of seeth nnetaaxsopli eayrll idngo eth smt.o fI hte enezym si bale to ncarisee ot lonamr ityvtaci itwh a giherh lelev of hte ebaus,ttsr hatt smane w’ere in a tvieeomtpci btoiinirh otnua.tsii Ctiepteiomv wsylaa cressso on eth ,gphar so we nca temianlie A dna .D
Now ew yaelnaz m.K We awnt lmsla sm’K for enttpo zenmy.se iezS eods ton asyawl trmeta 😉. etxpEc ni tish esca ew ’ndot tnaw ahtt ept,noyc ew awnt to ookl rof a 1e/(llary igb ,Km) laue(v thta is oslcer ot rzeo,) cb we wtna a ekaw ibg mK ot be aleb ot orrewevpo hte tmvoeipeitc oiibrtnhi. gpCraimno eth nnmargiei 2 ch,cseio B sah a mK elvau selcro ot erzo os htesre’ yuor ase.nwr
roF stih u,ietsnqo I saw tginknih taht eyht kadse uyo ocamepr to a “n”omalr roneps, so the xVma owl’utnd ghnace sncie ehty ettas ahtt shi ittaiycv runsrte ot a oarnml lvlee. lnOy teh -isaxx douwl cneis ti uwold eb ta sdneeciar ornetcnitconas in osc.mrpnoia
oS i onfud tsih tuesioqn ..rosildciuu. the soinxapletna vdredopi idd ont elalyr ceral ti pu ofr e,m btu i kithn i ddnnuretas it won easpe(l toerrcc me if im' )o.nwgr
iFrts nad r,motsofe Im' tno neve sreu if sith is yutrl a lib-evrernkuweea opt,l ncies teh Xs-ixa is otn 1Km./
tBu, rome lerlyange, I edtir to natrudends twha penpheda ta obht metresxe of PLP][ and lto.iyeVc
LPks:P A :usyfreol rwehe no eht aprhg is [PPL] hetigh?s Wll,e if teh xaXi-s is qelua ot PP1/,L PLP lwdou eb ta ist gthishe at eth aevul csoelst ot 0 no the a.-xisx :ntk(ih tahw si 1 vdidied yb a ?lbinloi YERV OCELS TO .R)OEZ iThs lowdu psnrodcreo ot a nopit onagl eht aixs-y rfo het znmsyee tciy.vtai nI oetrh wso,rd PPL is ta tsi sghthei ernioncnotcta ta het sghpra -tirenYetc.p
emmrebeR ahwt ew soal i:das hnew hte uaemttd nzemye is durnoa oslt fo P,PL it blsylciaa utnocfnsi lkei a nlm ze.mnye sTih mns,ae htat hte xmaV of eht aumdtte meeynz si uietnqaevl to teh xVma of teh rnomal eznyem. e,feerhTor The a1/Vxm fo obht nmzeyse rea ..enqtalev.iu at teh ipre T-eint!hytsc is bi!g fI oyu tge stih f,ra hnte uyo anc tmeiialne A)( and D(,) ecisn sehte agphrs eahv tnrefidef etpYenrsti-c tre(fedfni -x)smaVe rof iehrt ercepesvti enmy.zes
gonMiv .no..
lytiVceo ():V e hWn V is at tsi hietgsh (..ei )Vmax, the vluea fo 1V/ udowl eb at sti oletw.s :Aan(gi thnki fo ti ctay:rcilalp twah si 1 iddediv yb 1 l?iiblon ~!)0 ereohre,fT hte uavle of 1V/ is ta tsi osletw wehn eht ysai-x si elaqu ot .0 reTfo,erhe ehT mVxa fo sthi rccsou nwhe eth xi-yas si seolc ot o.zre.. eHrse the gntih ..tho etehs mnzeyes riqreeu tifefednr eeslvl of PLP to atpeero at amVx.- mbereem,R the -amVx fo teh MEADTTU ymezne lwli apehpn ta a mhuc IERHGH P]P.[L rO ln,rvoeyces the mV-xa fo eht AONMRL mzeeyn ilwl hpapne ta a uchm WEORL P[PL.]..
be eawra tath teh sa-ixx ofr this onsteuqi is not mK"" (tiBv6 is not het bh t'srtuteasat t1[i6.B]/V to fmiypsi, beusaec eth mxVa si nnaog tsay hte em,as uyo jstu eden to esanrice ]6[Bitv to gte eth maes Vaxm kaa( fstih to rihtg no the .x)ai-xs oD'tn be oolefd rnoadu yb teh tsiuoenq ewt.rri
bdNyoo sha wesernad tsih tqioeuns grtuhoh eht nles of a aelrngni viboecetj uiteq yet os sre'he ym 2 tsn:ce Km si IRDASNEEC cb isht ttiapen ash an nmeyez htiw an aovlrle LWROE nfifiyta cna( be cercedrot ot armonl feart an ceinsrae in es)ubttras ihchw si rtaeiacthircsc of ECIVTMPTEIO ib,nihtinio hhicw is ywh eht neli fo het .pt si noe fo tecptimevoi hnibiotiin no a BL tlop
ehT eoiqsutn si baylsclia isgttne pu a nituostai mlisira to that of a itcpioevetm ib.nirtohi yTeh sya ttah hihgre ncnrtoinsoatec fo lxyoiaprd athophpse scaesinre eht acittviy of hte enyzme ot nomlar lveel.s ,riamSiyll enhw yuo heva a eteocmvtipi hbiontiri dan eacrsein het cenctartoinon fo bu,atersst hte zymeen anc ieevcah hte esma Vmxa sa if ohuittw hte i.intrbioh
mRremeeb 1 ) ceivtpmteoi sobniithir ~ elisn Crsos ta cnt-esetry2)i niteo-Nptcoevim ibnishtrio ~ no lnei ssrco
nLgo swarne edha,a btu aerb thiw .em
IHTN: v soklo dikn of iekl ,y wrahese k slkoo ermo ikle .x
enrtpcyti-e = xaV1/m
rtip-etxecn = /m1K
teoN atht Vm,ax sa a uaserem of apmfe,onrecr cna eb reatled utoghhr ynam tn.hsgi M,eanehlwi mK is a ets iastrectihcarc of the mn,ezey dna oactnn eb daetle.r
In shit ex,elpma eth ymzeen onrpecamerf maV)x( is eaercsdni yb riasenngci eth nivtmia cfrtaoco so tath it recaseh a "n"rolam ivya.ttci Howrev,e eht eemzyn si lstli elyninerht htstyi edu to a tlngaionec tfedec, os teh mK stasy hte same.
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