GU!YS Yuo rlelay aveh to lkoo ta eth xsia sllaeb. hTe oeutsqni ssay teh ps.'t cstyoniiehatn ssethany itvaityc iaxy-)(s is LMNROA tiwh na SECAEDRNI otaumn fo iloxrdypa paphoehst (s)-ax.ix xa-Yis is het asem sa MRNL,AO isxa-x hfssti to eth rhgti oginswh RCEEIDANS xdoiplayr tsepohhap

ehT wnveaiereL Bkur tloP is mnalyi tinerretdep by sit X dna Y ciTrettnp .eseh iensqotu taetss thta vignig 6B aeecnssri eht yatitivc ot alnrmo elsv,le wihhc amens eth tyactivi soudlh eb hte smae sa het nrecm, aone.lH rhtei Y itcrnteep dholsu eb het emsa, so we era twnbeee chsceoi B dna C. A dna D do tno heva the aems Y cenritpte sa nilhe rmaoHrg. rcsonntieocant fo 6B ueacds atvyctii to eceomb olnram, dan so xmaV wlil nto be nnggicah nda ew acn caenlc A dna D .A hsa a woler xmaV adn D sha a hmcu hgiehr xmahTV.e efifnecred enbteew B and C si in rthie m.K gviMon to eth ftle on het X xsai emska teh mK lerow nad lstel uyo ttah ftiafniy si rhshog ei you oudlw ton dene reom 6.B utB in rou aces finitayf of hte znmeye orf B6 si elrlya ,wlo hhciw si hwy ew need a not erom 6.B

In raymsm,u ew anwt het maes axVm dan a hrehig K .meW tnwa the nrlom"a" vtyiicat (esma mxav as r)amoln adn ew dene irehgh onutsam fo 6B fro sseucsc intafsi yof of teh zenmey fro 6B is bopalbyr veyr lw.o

ioChce B sha a 1-(K/m) avlue eolrsc ot 0 which mneas Km is weorl dna itnaffiy of het ymzeen orf tis stesubrat is esurp hloisT w. askme sense as invgig ehhgri notumsa fo hte toptvmciee""i tbtssruea B6 is egh.lpni

iegNden hieg"rh "tooartniecnncs of hte B6 fro neemyz ttiivcay is otearhn ayw of ynsagi Km is iherhg iencs oerm is reeuidrq rfo /12 mvxa taciiyvt. Icneesrad mK seulav usretl ni 1/mK bineg alslmre rce(ols to 0 on eth x),aix-s hichw is rastnedtmeod ni swerna oheicc B.

A r"lman"o myeenz tiyitacv ni the cneseper fo hriehg B6 ntntecocosinar asnme ttha amVx si nto nhgagni,c tbu Km i.s

emSo fo sethe lxnataepoins reayll odign het tmos. fI eth meneyz is leab to aernscei to oarnlm atictivy hwit a rgeihh evlle of the taetsr,ubs taht measn ’reew in a ieemtotpicv thiobrini i.tsinutao Cpvtometiie syaalw cosesrs on the rpga,h os ew cna iaetminle A and D.

oNw ew yaaelzn mK. We awtn lmsla ’msK orf optten nzesye.m zieS odse ont asyalw metatr .😉 expEct in iths aesc ew o’ndt nwta atth ,potecny ew twan ot oolk fro a a/l(ryl1e igb Km,) (evalu htta si erlsoc to )ozr,e bc we nwat a kaew bgi mK ot be blea to rowoperve teh tietcmevopi rniti.bohi nprmoCgai het mgnainrie 2 chieosc, B hsa a mK uavel escrol ot oerz os ret’hse uyro are.wsn

For shit esnuoqit, I aws tkninhgi atth ehty deksa you pmcaeor to a a”r“omnl osn,per so eth axVm dwn’uolt ehngca neics yhet etast hatt ihs vtiictay rneruts to a mlorna elvl.e lOyn teh xi-asx dulwo inecs ti uldwo be ta esdicearn nrsnnaotcitcoe ni cmarsonoi.p

oS i udofn hist iestqonu lro.icud..siu het ieanptonsxla doverpid did nto lrlyea rcael ti up rof em, btu i khnit i unartednsd it won a(eselp ocrectr em fi i'm nwg.)or

srFit dna smtrefo,o I'm tno enev erus if iths si ruytl a ielabnvewuekr-er pol,t sinec hte sXaix- is nto 1K/.m

,tuB remo yae,lrgnel I drite to rndseaundt awth epnehadp ta obht resxmeet fo [P]LP nda lyico.tVe

kLPA:Ps olefyrs:u rwehe no eth prgah is [P]PL hetsh?gi Wlel, if teh i-saxX is uqeal ot /P,1LP LPP luodw eb at its ehhitgs at eth eulav scoetls ot 0 no eht s-axix. ik:(htn hwat si 1 ivdddei yb a nobl?ili REYV OLSCE TO R).OEZ Thsi wluod odcersonpr to a onpit gnlao eth -iayxs orf the ensmyez vitit.yca nI rohet d,srwo PLP si ta ist gsihhet nooncteinract at hte rgahsp Yt.neect-ipr

emebrmRe twha ew olas dais: enwh the mdttaue ezmeny si nroaud ltso of ,PPL ti yacibalsl cuotinsnf kiel a nlm ynm.zee hiTs asm,en hatt teh Vmxa fo the mdteaut zeymne si aiunlqteev to eht mVax fo hte amlnro yn.emez e,Tererhof Teh 1aV/xm of both mzysnee are viu.nt.el.eaq ta het i-r etptihysn!eTc si bgi! If oyu tge tsih fr,a enth yuo nca ilmaeinet A() dna D)(, csine ethse arghsp vhae neffrtedi Ysr-ttipenec (rftnfdeei e-xam)Vs rof their tieervecsp .mynseze

Mvoing .o.n.

tceoiyVl ):(V nehW V si ta tis tihghes i(..e axm,V) eth alveu of 1V/ dowlu be ta sit wtos.el ga(Ani: ktihn fo it rap:iylctlac ahtw si 1 idvdeid yb 1 ?libloni 0~)! eo,fheerTr hte aulev fo V1/ si ta sit leoswt ewhn hte -yisxa is alque ot .0 hf,eerroTe Teh mxaV of sith oscurc wnhe eht iyxas- is elcos to .r.oz.e eeHrs the ihgnt ..toh sehet eeyzsmn ruerqie refeftdni vleles fo PLP to eprateo at .xa-Vm emeRre,bm het xam-V of het EDUTMTA eyenzm wlil hppean at a hcmu RGHEIH .P[LP] rO v,renscolye het xVam- fo hte ARMLON myezne lwil pnpahe ta a mhcu OELRW PL.[P]..

eb erawa that eht sxi-xa fro hist suietnoq is not "K"m Bivt(6 is tno het aera uttsb'shtt [1B./Vit]6 ot ,iympfis aeceubs eth Vmax is anngo stya het ea,ms you tjus dnee to resineac t][6vBi to tge eht esam amVx aka( ftshi to tihgr no the .as-x)xi Dno' t be oelfod rdonau by eht oisqeunt re.witr

Noydob sha eewsdnra isht oqisnuet urhothg eht nsel fo a niagrenl beejctvoi itequ eyt so eesrh' my 2 s:enct Km is RESEDNCIA bc htsi aittenp sah an yzenme iwth an rvloela ROEWL antiyffi acn( eb rdoctceer ot arnmlo artfe na eeircnsa in eatst)sbur cwhih is crcishracattie of VTITIPOMEEC nhbiioi,tin wihch is hwy hte ieln of eht t.p si neo fo tceiveimpot hniibotini no a BL tlop

hTe qintsueo si claiasbly ttsgein up a iaoisntut aimrisl to ttah of a ttcvemopiei inhiti.obr Tehy ysa ahtt eghhir oinnrtctsenoca fo dixopyrla speaohthp rsceiensa the vitiycat fo teh zynmee ot alornm ls.evel iSi,rymlal ewhn oyu vhae a ieioptevmct itonibrhi and cnrseiea eth inccrotaotnne of stsbtaur,e the meneyz nca eviceah eth emas xVma sa fi howutti eht ibh.tnroii

eeRrmbem 1) eevticotmip isriinhbto ~ elnis rsCos at ne)ctsy 2ert-i ve-tctmNpoioine isinhrtoib ~ on elni rcsso

nLgo ewnars aah,de tbu aerb hiwt e.m

I:NHT v ksool dnki of iekl ,y eharwes k loosk omre ekli x.

eient-trpyc = /mxa1V

• xVma si the pperu liimt no owh tfas a aetrinoc is ydacazetl by .enmeszy

pentxrtcie- = 1K/m

• Km si a kgrnnia fo who good na ymeezn si ta dnginib tsi betss.tuar nA zmenye htwi a nnrgika fo 1 si teetbr ta igbnndi its stastubre ahtn an mzeeyn whit a nigknar fo .5 row(Le Km = eetbrt eye)zmn

Nteo atht ,mVax sa a emesaru of noaerprmf,ec anc eb atrleed hrthugo nyma .insgth eMhiwa,lne Km si a set tiarcairtchesc of eht mezye,n nad actonn be teel.rad

nI sthi x,eeplma het eezmny amerrcepfon (Vm)xa si sadcenire by csregniian het amivint oaoccrtf so atth ti hcersea a omrnl""a yciivtta. vw,Horee teh emyezn si lstil eyerlnniht itsyht due to a citanlgneo c,efdte os eht Km asyst eth .amse