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Retired NBME 15 Answers

nbme15/Block 3/Question#23 (reveal difficulty score)
An investigator who wishes to test the ...
Immunohistochemistry ๐Ÿ” / ๐Ÿ“บ / ๐ŸŒณ / ๐Ÿ“–
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submitted by โˆ—cassdawg(1781)
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Immunohistochemistry uses fluorescently tagged antibodies to bind specific antigens on tissues. In this case, an antibody could be directed against CD44v10 variant protein used to tag the cells in the samples that express the variant. This is also useful because it can be done on slides of paraffin embedded tissue.

  • Affinity column chromatography is used to separate a biochemical mixture based on interaction between antigen/antibody, enzyme/substrate, receptor/ligand, etc. but would not be useful in pre-embedded tissues or this case
  • Density gradient centrifugation is used on mixtures to separate them into components and is particularly useful in separating cell suspensions, such as in separating blood into layers to get the buffy coat with WBCs
  • Northern blot is for RNA and fluorescently tags a piece of RNA present in a sample
  • PCR is for identifying/anplifying a DNA segment
  • Reverse transcriptase (RT) PCR is typically used to measure expression levels of an mRNA
  • Southern blot is for identifying a DNA segment in a digest
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