Lomustine ia an alkylating nitrosourea compound used in chemotherapy. Alkylating agents crosslink DNA.
Cyclophosphamide metabolizes into a phosphoramide mustard. Phosphoramide mustard forms DNA crosslinks both between and within DNA strands
Cholchicine and Vinblastine work on microtubules
Methotrexate and 5-FU both work on purine metabolism.
Cytosine arabinoside interferes with the synthesis of DNA. Its mode of action is due to its rapid conversion into cytosine arabinoside triphosphate, which damages DNA when the cell cycle holds in the S phase (synthesis of DNA). Rapidly dividing cells, which require DNA replication for mitosis, are therefore most affected.