Immunohistochemistry uses fluorescently tagged antibodies to bind specific antigens on tissues. In this case, an antibody could be directed against CD44v10 variant protein used to tag the cells in the samples that express the variant. This is also useful because it can be done on slides of paraffin embedded tissue.
Affinity column chromatography is used to separate a biochemical mixture based on interaction between antigen/antibody, enzyme/substrate, receptor/ligand, etc. but would not be useful in pre-embedded tissues or this case
Density gradient centrifugation is used on mixtures to separate them into components and is particularly useful in separating cell suspensions, such as in separating blood into layers to get the buffy coat with WBCs
Northern blot is for RNA and fluorescently tags a piece of RNA present in a sample
PCR is for identifying/anplifying a DNA segment
Reverse transcriptase (RT) PCR is typically used to measure expression levels of an mRNA
Southern blot is for identifying a DNA segment in a digest
submitted by โcassdawg(1780)
Immunohistochemistry uses fluorescently tagged antibodies to bind specific antigens on tissues. In this case, an antibody could be directed against CD44v10 variant protein used to tag the cells in the samples that express the variant. This is also useful because it can be done on slides of paraffin embedded tissue.