ehT veaiwnreeL urBk tolP si yniaml eidprnrteet by tsi X and Y Tec i.ehttnersp inqoseut ssteat ttha vgngii B6 aieescrns the taiytivc ot nmralo s,velle iwchh nsaem het atitvcyi udhsol eb eth msae as eth lneo.rH c,enma itrhe Y cptieretn sludoh eb the s,mea os we ear bteenew sihceoc B and .C A and D do tno evha het aesm Y cenprteit as iaohnHr merg.l etncnrcsotnaio of B6 cauesd taciiyvt ot obceme nrmalo, nda so axVm lilw ont eb cgngnhia dan we acn cclnea A dan .A D ahs a weolr Vxma nad D sha a hmuc reihhg mVaex .hT fceeenrdif eewtnbe B nad C si in hteri .Km vMnoig to the telf no het X ixas ksame the Km owerl and ellst yuo thta ayfiitfn si hiosregh yuo dwolu ont ened emro B.6 But ni uor sace ffyaiitn of hte ynemez orf B6 si lryael wl,o ihhwc si yhw we need a ont oemr .B6
In amrmsy,u ew atnw hte esam aVxm adn a ghhrei e KWm. atnw teh "lroa"nm ytitvaci (smea mxva as oal)rmn nda we ened ehrhgi suatmno fo 6B fro sccusse oiatyfsifn fo the yeemnz orf 6B is baboyrlp eyvr low.
iehcoC B has a m/1K)(- uveal reclso to 0 chwih means mK si rloew and tfnaifiy fo eth eeynmz rfo tsi usrttseba is sprue iTso.lwh maeks essne as ivngig erihgh mansuto of teh "eimeiv"tpcto attersbsu B6 is nhpl.eig
ngNiede ghei"rh rnotcn"aeisncot of eth 6B ofr ymneez taiyvtci is ahontre way fo aginys mK is ehhigr incse roem si uqirdree rof /21 vamx ctytviia. eacerIsnd mK lvaeus eslutr ni /m1K iebng mearsll r(oelcs to 0 on the ,)x-ixsa hwhic si etnoddmestra ni aesrwn ccieho B.
A r"ml"oan yeeznm ictiytva ni hte nsceepre of ieghhr B6 rsonnoecnittac ensma ahtt axmV is not ggiac,nnh utb mK si.
Semo of eehts xnslaontaepi yaellr dogin hte ts.mo If the znemey is able to srieaecn to lrnmoa iitatvcy ihwt a ihrheg leelv of teh es,rstutab ttah nemsa eewr’ ni a ipmevtiecot nobhtirii auitins.ot Coeipitmtev ywsaal csosers no the rh,pag os we acn ianeleimt A dna D.
oNw ew aelyzan m.K We antw aslml m’Ks rof totnep msyne.ez Siez sdeo otn lwasay ratemt .😉 ectpxE ni sith csae ew dtno’ tawn ttah peyotcn, we awnt ot kolo ofr a (/yelarl1 igb ,mK) ue(vla thta is csrelo to ,eor)z bc ew atwn a kwae gbi mK ot be ebla ot revowpore teh vetiipomcet tirhonbi.i oarignCpm eht giinemnra 2 ,ccseioh B ash a mK uavle lseorc ot ozre os trhsee’ yoru ra.nsew
orF siht nu,osiqte I wsa tgiiknhn atht htye eakds you rmepaoc ot a lmona”r“ esr,nop os eth Vmax udn’lotw neghac sneic yeth etsat tath hsi iiycvtta srertnu to a oarmnl vele.l nOly teh i-xaxs loudw nceis it ldowu eb at dnrcseeai tnnaisencorotc in posirac.onm
oS i unodf hist ietsuqno c.su..duroili eht sanpeaiolntx dvoperdi did not llryea aercl ti pu orf ,me ubt i tkhni i rusnntddea it own le(apse cterocr me if 'mi .ngwro)
irFts and ,ooftmrse mI' ton nvee user if tish si tyrul a eiuvleekbera-rwn lotp, cnsie hte -Xsxai is tno .1m/K
,But more leyanelrg, I tride to rtndadusen ahwt enpdhpae ta otbh rxteeesm fo P]PL[ nda eotliV.cy
APL P:sk osluefr:y eerhw on eth grhap is PLP[] theish?g elW,l if eth sai-Xx si lequa ot ,/1PLP PLP dwuol eb ta sti ghsthei at the lvaue stcsole ot 0 no the xa-six. (nkht:i twah is 1 ideivdd yb a nbillio? VERY SOLEC TO Z.O)RE iTsh lduow esprdncoro to a otinp galno eth i-ysax fro teh eysmzne yvciat.it nI hrteo or,swd PPL si ta ist hhtgesi cnoinetcnaort at teh hrasgp ceep-trt.Yni
rmebeeRm thwa ew osal siad: henw eth edmautt nmeezy si uanodr otls of LP,P it calybsila scnnitufo ilke a lnm eyenz.m Tshi meas,n ttha teh mxVa of teh uttmead enzyme si evenuitalq to hte axmV of eht ralnom e.myenz ehfTrr,eeo The /Vm1ax of othb ymeznse era .venltaie.qu. ta the erhispc!ei n-tyTt si !gib fI uoy teg hits ,fra hnet oyu nac inaleeitm A)( nad D(,) sceni etehs ghspra hvea ertfnfedi ietYecpsnt-r fetre(fndi xV)emsa- fro irhte tevsrecpie eynsmz.e
Mnivgo .on..
tlVceioy (:V) eh nW V is ta sti gshethi ..ie( )mVa,x the auevl fo /1V ldwou eb ta tsi el.ostw A:(niga itnhk fo it calcatpir:yl ahwt is 1 ideddiv yb 1 ii?lbnol )0!~ hTeofr,ree teh evalu fo 1V/ si at ist otlews nweh het ysxia- si elaqu to 0. ,orreeThef Teh mVxa of tish crsouc hewn eth -iyxsa si losec to ..eo.rz sreHe hte ntgih ..hto tshee msyzeen qrrueie fefrdnite lveels fo PPL to trepoae at V.xa-m ,mrbeeRem eht xV-ma fo the AMDETTU eymzen wlil epahpn at a mhuc GIHHRE [.P]PL Or srnec,yvoel het Vxma- fo eht ANOMLR emnyze wlil epanph ta a uchm EROWL LP.P.][.
eb awaer ahtt hte i-xxsa rof this tqsoiuen is not "m"K Bti(v6 si ton eht aruht bt'asttse [.6iV/]tB1 to isipyf,m ucaseeb eht xmaV is nnago ysta the as,me oyu utsj eden ot inseerca t]6ivB[ to egt hte amse aVmx (kaa htifs to tirgh no teh sxxi)a-. nD o't be oedolf uardon by teh qusitneo er.wirt
odbNyo ash erdnwsea hits tosineuq urohhtg eth elsn of a eglniarn ejibvetoc tiqeu ety so reh'es my 2 nc:set m K si NDIEASCER bc siht aittnep ash an ezenym twhi an lovlear LEWRO tiiyfnaf (anc eb eetrcodcr to normal treaf an incseear in at)sbsteur wichh is esitrchticcaar of PIVTIMEOTEC ioniitin,hb hwich is hwy eth eiln of teh .tp is one fo vieimeoptct nthnobiiii on a LB potl
ehT otuenqis is lalaisbcy gsenitt up a uiiostnta imrasli ot thta fo a cpoimivttee .noiibriht eyTh yas atht egihrh ioetanctsrnonc of aidyrpoxl ahohsptpe acnesries eht cvttiiya of hte zymene to aromln esl.evl yaSll,imir wneh yuo ehav a ipoicettmev hriiotnib nad nesrceia het riotaconcnent fo ,seustbrta het mezeyn anc vchaeie the smae mVax as if uhwotit het nitirboh.i
eeebmRrm )1 opmieevttci hsitiinobr ~ slnie ssoCr at ect)-tinesry2 oc-oietmpivNnte bhsnioiirt ~ no neil rcoss
nLgo ensawr d,aaeh utb reab ihwt .em
IT:NH v lokso dnik of eikl ,y weerhas k lkoos mreo keil .x
ce-ienpttry = amxV1/
ei-pxtnretc = 1m/K
Neot ttah mxa,V as a eurmeas fo e,rcfnoaremp anc eb erltdea rhoghtu yman nthis.g nwl,hieeMa Km si a ets arhitcctecrsai fo eth nzem,ey nad nnatoc be .terelda
nI tshi lem,eapx het yemzen enafmorpcre )amVx( is iscaender by ecrnaigisn eth imivatn cacrofot so that it ascereh a n"oaml"r .tacvtiyi eoH,ewvr hte zemyne is illst riyeehnnlt yhtist deu ot a icnanglote fdtece, so eth mK ytsas hte mae.s
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